Kuzvidzivirira kwePoldacrylwide gel Electrophoresis

· PolwandacrrylambideGel Inofanirwa naGel Norrylamide Monomer, Polymerization Kutanga zvinyorwa, Catalyst, uye kodzero yemunyu uye buffer musanganiswa pamwe chete.

· acrylamideuye bis (n, n 'n' - methylene Double Acryleride) ndiye Monomer fomu gel matrix.

· Ammonium inofadza tanga kujairira polymerization maitiro. Kuumbwa kweglue kunoda 10% imbimammonium yekupedza mhinduro yakagadzirirwa mumvura. Ruzivo rwakawanda rwakaratidza kudiwa kwekushandiswa kwakashanda. Nekudaro, iyo 10% mhinduro inogona kuiswa pa4】 kwemavhiki akati wandei pasina kukosha kwekuita kwebasa. Gadzira kusvika pagumi ml uye kurasa kana glue inotadza kuunganidzwa.

ZANO: muzana yeacrylamideMukutsakatisa glue uye protein glue haina kufanana. Kana uchishandisa premade acrylamide: Bis solution, ita shuwa kuti uwane iyo chaiyo bhodhoro.

Temiger (n, n, n 'n', n 'n' - tetramethyl ethylentiamine) ndeyekutonga, muhombodo yebhuruu, yakaiswa mufiriji. Wedzera chete usati wadurura glue.

· Polacrylwide Electrophoresis inoshandiswa girazi mumagetsi dzinofanira kushambidzwa nguva dzese uye mushure mega nguva. Mushure mekunge Electrophoresis, geza nejira nyoro uye jira mumvura inopisa sofa, geza nemvura yakadzika uye kumira kuti iome.

· Zvinyoro uye guruva zvinogona kukonzera nehwanda polymer. Usati wasvitsa electrophoresis, suka iyo girazi ndiro negirazi kuchenesa uye kupukuta nejira nyoro. Geza nemvura yakadzikira uye yakaoma kwazvo nepepa kupukuta. Rinsing ne 70% ethanol usati wapukuta nepepa anobatsira kuchenesa uye kukurumidza kuoma. Wedzera masampula eAcrylamide zvichitakura: BIS, mvura, buffer solution, imbifomium inofadza, temed. Zunza zvakanaka uye udurure pakarepo.

Izvo hazvidikanwe kuti degass polycrylwide pamberi pePolymerization. .

Yakatwasuka glue point sampuli matipi.

Isa chidimbu chepepa dema mubhokisi repazasi, dema kumashure kuti uite imwe gomba rekuratidzira kuti uone zvakajeka.
· Glue tank yakazadza buffer, pamusoro peiyo colloid.
If Kana mupendero une mwenje, tendeukira magetsi, rega chiedza chivheneke. Dhirowa sampu mu pipette.
· Sere otomatiki pombige mudziyo.
· 7 ebe · negumi mu 1 memagetsi ekufambisa nzvimbo inogona kushandiswa mune yakawanda yezvinyorwa pane sampuli. Zvemabheburu madiki emuviri (asingasviki 10μ1), iyo refu pipette musoro inoshandiswa kutevedzana glue iri nyore nyore.

· Pipetting yakangonyudzwa mumuenzaniso, inhaling zvishoma nezvishoma fluid. Muenzaniso unogona kuoneka anonamira ne glycerin, uye nekukurumidza kupomba kunogona kudhirowa mhepo mabhuru mumusoro wepaipi.

· Samples mushure mekunakidzwa kwemusoro pipetting musoro, uchafambisa mvura yemvura zvinyoronyoro pamucheto pombi kana kupukuta pepa rinoyamwa musoro kunze kwemakonzo. Chenjera kuti urege kuyamwisa muenzaniso.

Isa muenzaniso mumuenzaniso mugomba remuenzaniso

· Pipetting mudziyo wekuti uchengetedze kumanikidzwa zvishoma, ita kuti samples zvishoma kufamba-famba mumusoro wemvura.
· · Iyo pombetting musoro yakaisa mu buffer, yakakwira zvishoma kupfuura nzvimbo dzemakomba, chengetedza kudzvanywa kwakanaka. Iyo tip yePipieti inogona kuiswa mugomba diki.
· Sref yadya uye zvishoma nezvishoma samples kunze. Iyo pipeette tip inoiswa pamusoro peiyo point sampule gomba, uye sampula ichanyura mugomba. Rega sampula inyungunure kuzadza gomba rekuenzanisa pane kusundira mukati.
· Kara wekupedzisira wekudonha kwemuenzaniso nemusoro wemvura, mvura ichaenda kugumbo rechipiri, zvishoma nezvishoma simudza fambisa mvura, buda kunze kweBuffer

Maitiro Ekuita Vertical Glue Sampling?

· Yakavambwa Glue Point sampula maburi akaumbwa pakati pezvikamu zviviri zvegirazi. Mune yakaonda kwazvo glue, iyo pipette musoro haigone kuiswa pakati pemapuranga maviri egirazi. Tarisa glycerin! Isa iyo pipette musoro pamusoro pegomba remuenzaniso uye semuenzaniso uchanyura mugomba.
· Pfungwa Sampula zvisati zvaitika, iva nechokwadi chekuisa iyo nzvimbo yePolypropylene ANYL Gel Sampula gomba rakachena. Geza lehmitized acrylamide uye mvura inogona kuoneka pazasi pegomba remuenzaniso. Mvura inogona kuita kuti gomba remuenzaniso idiki zvakanyanya. Shandisa 25ml kana 50ml syringe uye single 18-geji. Dururira mumagetsi eElectrophoresis buffer uye nekuchenjerera gomba rekuenzanisira.
· Zvingave zvakaoma kuona gomba rekuenzanisira, asi zvimwe chete, vamwe vese vari nyore. Kana paine makomba akawandisa, iyo sampula buffer inogona kuedzwa ne bromophephenol bhuruu.